The Diffley Lab
Chromosome Replication
Selected primary literature
2023
FEB
2023
Masashi Minamino, Celine Bouchoux, Berta Canal, John Diffley, Frank Uhlmann
JAN
2023
Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication
Jingkun Zeng, Stephanie Hills, Eiko Ozono, John Diffley
Cell. DOI: 10.1016/j.cell.2022.12.036
2022
JUN
2022
Mechanism of replication origin melting nucleated by CMG helicase assembly
Jacob Lewis, Marta Gross, Joana Sousa, Sarah Henrikus, Julia Greiwe, Andrea Nans, John Diffley, Alessandro Costa
Nature. DOI: 10.1038/s41586-022-04829-4
2021
DEC
2021
Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase
Julia Greiwe, Thomas Miller, Julia Locke, Fabrizio Martino, Steven Howell, Anne Schreiber, Andrea Nans, John Diffley, Alessandro Costa
Nature Structural and Molecular Biology. DOI: 10.1038/s41594-021-00698-z
AUG
2021
Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation
Allison McClure, John Diffley
eLife. DOI: 10.7554/eLife.69726
JUN
2021
Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro
Max Douglas, John Diffley
Nucleic Acids Research. DOI: 10.1093/nar/gkab416
2019
NOV
2019
Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM
Thomas Miller, Julia Locke, Julia Greiwe, John Diffley, Alessandro Costa
Nature. DOI: 10.1038/s41586-019-1768-0
FEB
2019
Rpd3L contributes to the DNA damage sensitivity of Saccharomyces cerevisiae checkpoint mutants
Belén Gómez-González, Harshil Patel, Anne Early, John Diffley
Genetics. DOI: 10.1534/genetics.118.301817
NOV
2018
Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisomereplication in vitro
Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa
Nature Communications. DOI: 10.1038/s41467-018-07417-1
2018
MAR
2018
The mechanism of eukaryotic CMG helicase activation
Max Douglas, Ferdos Abid Ali, Alessandro Costa, John Diffley
Nature. DOI: 10.1038/nature25787
2017
DEC 2017
Cryo-EM structure of a licensed DNA replication origin
Ferdos Abid Ali, Max Douglas, Julia Locke, Valerie Pye, Andrea Nans, John Diffley, Alessandro Costa
Nature Communications. DOI: 10.1038/s41467-017-02389-0
JUL 2017
Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading
Gideon Coster, John Diffley
Science. DOI: 10.1126/science.aan0063
JAN 2017
Chromatin controls DNA replication origin selection, lagging-strand synthesis, and replication fork rates
Christoph F Kurat, Joseph TP Yeeles, Harshil Patel, Anne Early, John Diffley
JAN 2017
How the eukaryotic replisome achieves rapid and efficient DNA replication
Joseph TP Yeeles, Agnieska Janska, Anne Early, John Diffley
2016
MAY 2016
Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation
Tom D Deegan, Joseph TP Yeeles, John Diffley
EMBO Journal. DOI: 10.15252/embj.201593552
JUL 2017
Recruitment of Mcm10 to sites of replication initiation requires direct binding to the minichromosome maintenance (MCM) complex
Max Douglas, John Diffley
2015
MAR 2015
Regulated eukaryotic DNA replication origin firing with purified proteins
Joseph TP Yeeles, Tom D Deegan, Agnieszka Janska, Anne Early, John Diffley
Nature. DOI: 10.1038/nature14285
2014
SEP 2014
Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase
Gideon Coster, Jordi Frigola, Fabienne Beuron, Edward P Morris, John Diffley
Molecular Cell. DOI: 10.1016/j.molcel.2014.06.034
SEP 2014
Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication
Kin Fan On, Fabienne Beuron, David Frith, Bram Snijders, Edward P Morris, John Diffley
EMBO Journal. DOI: 10.1002/embj.201387369
2013
MAY 2013
Identification of a heteromeric complex that promotes DNA replication origin firing in human cells
Dominik Boos, Mona Yekezare, John Diffley
Science. DOI: 10.1126/science.1237448
MAR 2013
ATPase-dependent quality control of DNA replication origin licensing
Jordi Frigola, Dirk Remus, Amina Mehanna, John Diffley
Nature. DOI: 10.1038/nature11920
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