top of page

Selected primary literature

2023

JAN

2023

Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication

Jingkun Zeng, Stephanie Hills, Eiko Ozono, John Diffley

2022

JUN

2022

Mechanism of replication origin melting nucleated by CMG helicase assembly

Jacob Lewis, Marta Gross, Joana Sousa, Sarah Henrikus, Julia Greiwe, Andrea Nans, John Diffley, Alessandro Costa

2021

DEC

2021

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase

Julia Greiwe, Thomas Miller, Julia Locke, Fabrizio Martino, Steven Howell, Anne Schreiber, Andrea Nans, John Diffley, Alessandro Costa

Nature Structural and Molecular Biology. DOI: 10.1038/s41594-021-00698-z

AUG

2021

Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation

Allison McClure, John Diffley

JUN

2021

Budding yeast Rap1, but not telomeric DNA, is inhibitory for multiple stages of DNA replication in vitro

Max Douglas, John Diffley

Nucleic Acids Research. DOI: 10.1093/nar/gkab416

2019

NOV

2019

Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM

Thomas Miller, Julia Locke, Julia Greiwe, John Diffley, Alessandro Costa

FEB

2019

Rpd3L contributes to the DNA damage sensitivity of Saccharomyces cerevisiae checkpoint mutants

Belén Gómez-González, Harshil Patel, Anne Early, John Diffley

NOV

2018

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisomereplication in vitro

Panchali Goswami, Ferdos Abid Ali, Max E. Douglas, Julia Locke, Andrew Purkiss, Agnieszka Janska, Patrik Eickhoff, Anne Early, Andrea Nans, Alan M. C. Cheung, John F. X. Diffley, Alessandro Costa

Nature Communications. DOI: 10.1038/s41467-018-07417-1

2018

MAR

2018

The mechanism of eukaryotic CMG helicase activation

Max Douglas, Ferdos Abid Ali, Alessandro Costa, John Diffley

Nature. DOI: 10.1038/nature25787

2017

DEC 2017

Cryo-EM structure of a licensed DNA replication origin

Ferdos Abid Ali, Max Douglas, Julia Locke, Valerie Pye, Andrea Nans, John Diffley, Alessandro Costa

Nature Communications. DOI: 10.1038/s41467-017-02389-0

JUL 2017

Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading

Gideon Coster, John Diffley

JAN 2017

Chromatin controls DNA replication origin selection, lagging-strand synthesis, and replication fork rates

Christoph F Kurat, Joseph TP Yeeles, Harshil Patel, Anne Early, John Diffley

JAN 2017

How the eukaryotic replisome achieves rapid and efficient DNA replication

Joseph TP Yeeles, Agnieska Janska, Anne Early, John Diffley

2016

MAY 2016

Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation

Tom D Deegan, Joseph TP Yeeles, John Diffley

EMBO Journal. DOI: 10.15252/embj.201593552

JUL 2017

Recruitment of Mcm10 to sites of replication initiation requires direct binding to the minichromosome maintenance (MCM) complex

Max Douglas, John Diffley

2015

MAR 2015

Regulated eukaryotic DNA replication origin firing with purified proteins

Joseph TP Yeeles, Tom D Deegan, Agnieszka Janska, Anne Early, John Diffley

Nature. DOI: 10.1038/nature14285

2014

SEP 2014

Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase

Gideon Coster, Jordi Frigola, Fabienne Beuron, Edward P Morris, John Diffley

Molecular Cell. DOI: 10.1016/j.molcel.2014.06.034

SEP 2014

Prereplicative complexes assembled in vitro support origin-dependent and independent DNA replication

Kin Fan On, Fabienne Beuron, David Frith, Bram Snijders, Edward P Morris, John Diffley

EMBO Journal. DOI: 10.1002/embj.201387369

2013

MAY 2013

Identification of a heteromeric complex that promotes DNA replication origin firing in human cells

Dominik Boos, Mona Yekezare, John Diffley

MAR 2013

ATPase-dependent quality control of DNA replication origin licensing

Jordi Frigola, Dirk Remus, Amina Mehanna, John Diffley

Nature. DOI: 10.1038/nature11920

We are funded by

The_Francis_Crick_Institute_logo_edited.
European_Research_Council_logo.svg.png
EMBO_logo-primary_black-digital.webp
marie_curie-logo-400px.jpg
12121212-1.jpg
Wellcome_Trust_logo.svg.png
bottom of page